Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical)

Endophytics Bacteria from Pangi Fruit (Pangium edule REINW.) and Its Potential as Producer of Antibacterial Compound

Natalia V. Sambuaga — 2024-10-30

Natural antibacterials can be derived from various types of plants, one of which is the Pangi plant (Pangium edule REINW.). Pangi fruit contains an antioxidant and antibacterial compound that can inhibit bacterial growth. This study aims to determine the isolates of endophytic bacteria contained in Pangi fruit and its potential as a producer of antibacterial compounds against Escherichia coli and Staphylococcus aureus bacteria. This study used exploratory description research method. Results Isolation research of endophytic bacteria from Pangi fruit obtained seven isolates. Each Isolate has a different morphology both from the shape, color, edges, and colony elevation. Based on the results of antibacterial activity testing, each isolate of endophytic bacteria showed different clear zone diameters. Endophytic bacteria showed different clear zone diameters against E. coli (0.75 mm – 4.5 mm) and S. aureus (1 mm – 5 mm). Endophytic bacterial isolates That have the potential as antibacterial with the largest clear zone diameter are Isolate PA 2.1 (4.5 mm) against E. c oli and isolate PA 3.2 (5 mm) against the bacteria S. aureus. Based on the results of morphological characterization of isolate PA 1.1 has similarities with the genus Basillus sp., isolates PA 1.2, PA 1.3 PA 2.2, PA 3.1 and PA 3.2 have similarity with the genus Basillus sp. PA 3.2 has similarities with the genus Paracoccus alcaliphilus and isolate PA 2.1 has similarities with the genus Alcaligenes sp.

Antioxidant Activity of Lotion From Sonneratia caseolaris Leaves and Trigona sp. Propolis Extract

Annisa Nurul Safitri — 2024-10-30

Daily activities can trigger free radicals due to air pollution and increase the decline in skin health. One of the prevention efforts by using a lotion that made from natural ingredients. Natural ingredients such as Sonneratia caseolaris leaves and Trigona sp. bee propolis are still limited used in the form of lotion combination preparations. The purpose of this study was to explore the antioxidant activity of a combination lotion of S. caseolaris leaves and Trigona sp. bee propolis. Antioxidant activity was detemine by used the DPPH technique with lotion formula combination of S. caseolaris leaves and Trigona sp. bee propolis. F1 (1:1), F2 (2:1) and F3 (1:2) accompanied by a physical stability test of the lotion. The results showed that preparations F1, F2, F3 had strong antioxidants in the concentration range of 60-100 ppm. The three formulas met the criteria for a stable lotion preparation. The combination lotion formula has potential in the development of cosmetic products from natural ingredients.

Antibacterial Activity Test of Sonneratia alba Mangrove Root Infusa Against Propionibacterium acnes and Salmonella typhi Bacteria

Andriany M. Christin — 2024-10-30

Infectious diseases can be caused by microbiological agents, namely bacteria. The ability of bacteria to invade and cause infection is referred to as a pathogenicity. The results of phytochemical screening produced secondary metabolite compounds alkaloids, flavonoids, tannins, saponins, phenolics that act as antibacterial. This study aims to determine the antibacterial activity of Sonneratia alba mangrove root infusa against Propionibacterium acnes and Salmonella typhi bacteria. This type of research is a laboratory experimental research, using the well method by doing three repetitions on 5 concentration series, 1 positive control and 1 negative control for two types of bacteria namely Propionibacterium acnes and Salmonella typhi. The results obtained from the Kruskal Wallis test on Propionibacterium acnes bacteria are Sig 0.028 < 0.05, and the results of the ANOVA test on Salmonella typhi bacteria from Sig = 0.000 < 0.05. And it is known in Propionibacterium acnes bacteria that 25% concentration inhibits 5.83 mm, 50% concentration inhibits 6.3 mm, 100% concentration inhibits 9.43 mm. And in Salmonella typhi bacteria that concentration is known that 25% concentration inhibits 9.73 mm, 50% concentration inhibits 9.43 mm, 100% concentration inhibits 11.57 mm. Based on the results of the study, it can be concluded that Sonneratia alba root infusa has antibacterial activity on Propionibacterium acnes and Salmonella typhii bacteria.

Antibacterial Activity Of Staphylococcus aureus From Chinese Betel Leaf Juice (Peperomia pellucida L.)

Kezia T. Wantah — 2024-10-30

Infectious diseases, especially in developing countries such as Indonesia, are a common health problem, one of which is caused by bacteria. This study tested the antibacterial activity of Chinese betel leaf extract against Staphylococcus aureus bacteria, with secondary metabolite content such as alkaloids, tannins, and flavonoids that act as antibacterials. This study was a laboratory experiment using a completely randomized design with three repetitions at five concentrations of extract, as well as one positive and negative control. The method used was the agar diffusion method, and the test results showed that Chinese betel leaf extract had antibacterial potential against Staphylococcus aureus at various concentrations tested. The concentrations used were 6µL/disc, 8µL/disc, 10µL/disc, 12µL/disc, and 14µL/disc, where all these categories produced inhibition zones included in the moderate category according to Susanto (2012). Statistical analysis using the Kruskal-Wallis test produced a significant value of 0.035 (<0.05), indicating a significant difference between each concentration tested. From the research results, it can be concluded that Chinese betel leaf extract has effective antibacterial activity against Staphylococcus aureus.

Antibacterial Activity Test Of Epazote Leaf Infusion (Dysphania ambrosioides L.) Against Escherichia coli Bacteria

Saroinsong F. Carolina — 2024-10-30

The most common health problem in Indonesia is bacterial infection. Dysphania ambrosioides L., also known as epazote leaves, is an herbal plant that has antimicrobial potential. This research was conducted to see at what concentration there is antibacterial activity of epazote leaf infusion Dysphania ambrosioides L. against Escherichia coli bacteria. This laboratory experimental study used a complete randomized design (RAL) research. Antibacterial activity testing of epazote leaves was carried out using the disc paper diffusion method with concentrations of 50%, 75%, and 100%. Epazote leaves are boiled in an infusion pan for 15 minutes, until the temperature reaches 90 degrees Celsius. Ampicillin positive control 10 ?g/disc. Based on the test results of secondary metabolites of positive epazote leaf infusion there are alkaloids, flavonoids, tannins, saponins, triterpenoids, and phenolics. The results of the study showed that infusion of epazote leaves inhibited Escherichia coli bacteria by diameter inhibition. Average at a concentration of 50% = 14.04 mm, 75% = 16.92 mm, 100% = 17.94 mm, which means that there is antibacterial activity of epazote leaf infusion against Escherichia coli bacteria.